WATERS Webinar: Analyzing Viral Glycoproteins with MALS
Upcoming Webinar: Analysis of Ebola Virus Glycoproteins in Complex with Antibodies Using Light Scattering
Wednesday, September 24
18:00 - 19:00 Greek Time
Overview
Viral glycoproteins (GPs) are essential for the attachment and fusion of virus and host cell membranes. It is also the primary target of neutralizing and therapeutic antibody responses. Many viral GPs are found as a trimeric complex on the viral surface. Whether multiple antibodies bind to these complexes and with what affinity is critical information when designing vaccines or creating a post-exposure antibody treatments.
This webcast will explain how we characterize glycan content and oligomerization state of multimeric viral glycoproteins using SEC-MALS and demonstrate the detailed information we can gain about GP-antibody interactions using composition-gradient multi-angle light scattering (CG-MALS). We will also address the preparation of proteins for light-scattering studies as well as the set-up of the instruments and analysis of the data. After this webinar, Wyatt light scattering instrument users will be able to set up and perform their own SEC-MALS and CG-MALS experiments and critically evaluate the resulting data.
Key Learning Objectives
- Understanding the latest Ebola virus treatment and research
- Absolute mass analysis of glycoproteins using SEC-MALS
- Affinity and stoichiometry of antibody binding to multivalent glycoproteins
Presented By
Kathryn Hastie, Ph.D.
Instructor & Director of Antibody Discovery
La Jolla Institute for Immunology
Dr. Hastie uses protein engineering and high-resolution structural analysis to design better therapeutics and vaccines against viruses that threaten global health. She is a leading expert on viral glycoproteins and their antigenic landscapes, marrying structural insights and vaccine design. Dr. Hastie further spearheads antibody discovery efforts at La Jolla Institute for Immunology to find antibodies against emerging viruses and other therapeutic targets.
If you are unable to attend, you can still register to receive a link to watch the webinar on-demand after its initial broadcast.