Characterizing and Monitoring Lipid Nanoparticle Impurities Using the BioAccord LC-MS System with waters_connect Software

Lipid nanoparticles (LNP) have been increasingly used with high efficacy as delivery vehicles for nucleic acid-based therapeutics and vaccines. However, the components of these lipid shells can often contain impurities in addition to the core lipid components.

Impurity analysis is a critical aspect of screening lipid raw materials and compositional profiling analysis of the assembled LNP delivery system. Download our recent app note to learn how the BioAccord LC-MS System and waters_connect informatics workflows streamline the routine analysis of LNP composition and facilitate identification of component impurities.

Already a waters_connect User? Access a newly released data and training package for lipid nanoparticle and LNP impurity analysis.

If you’re already a waters_connect user, there is a new data and training package, available at no cost, that allows you to investigate this application further. Your system manager can obtain these methods and training files through the digital software delivery system. If you have questions, please contact your local Waters Account Manager.

The methods and example data sets illustrate the analysis of four key components that comprise lipid nanoparticles as well as detecting impurities of those components:

§  Ionizable Lipids

§  Cholesterol

§  Neutral Phospholipids

§  PEGylated Lipids